HIV Testing

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HIV Testing

Since the early days of the HIV epidemic, HIV tests have improved considerably. We now have multiple methods to test for HIV infection, and today’s testing technologies are getting better at detecting new infections. This has led some HIV testing experts to suggest that we can detect the vast majority of HIV infections much earlier than many people realize.


Why is it valuable to detect HIV infections as early as possible?

There are two major reasons why it is valuable to detect an HIV infection as soon as possible after it has occurred:

Early detection is good for people getting tested for HIV. HIV tests that provide an accurate result sooner after infection may significantly reduce the anxiety of “not knowing” that many people feel after they think they may have been exposed to HIV.

For those who test HIV-positive, testing early may give them a better sense of how and when they were exposed to HIV. It may also provide them with greater opportunities to access services and support that will help manage their health and well-being. Another distinct advantage of early diagnosis is that people can access anti-HIV treatment before their immune systems have been severely damaged, which can also improve their long-term health outcomes and help prevent HIV transmission.

In fact, the evidence for sexual transmission shows that people on ART who maintain an undetectable viral load do not pass HIV to their sexual partners.

Early detection can help prevent new HIV infections. Research demonstrates that almost half of new HIV infections may come from individuals who have been newly infected. This may be because people who are newly infected have significantly higher levels of the virus in their blood and genital tracts, which may make HIV transmission more likely to occur. People who are newly infected are also more likely to be unaware of their HIV status. Diagnosing HIV infection early allows a person to make more informed decisions (such as practicing safer sex and using drugs in a safer way). Research shows that when aware of their status, most HIV-positive people do take steps to prevent HIV transmission.

Being able to detect new HIV infections earlier is not only beneficial to people seeking testing and to the service providers offering testing services, but may also play a significant role in preventing further transmission of HIV within the population.


HIV testing should be voluntary and the right to decline testing should be recognized. Mandatory or coerced testing by a health-care provider, authority or by a partner or family member is not acceptable as it undermines good public health practice and infringes on human rights.

Some countries have introduced, or are considering, self-testing as an additional option. HIV self-testing is a process whereby a person who wants to know his or her HIV status collects a specimen, performs a test and interprets the test results in private. HIV self-testing does not provide a definitive diagnosis; instead, it is an initial test which requires further testing by a health worker using a national validated testing algorithm.

All HIV testing services must include the 5 C’s recommended by WHO: informed Consent, Confidentiality, Counselling, Correct test results and Connection (linkage to care, treatment and other services).



If you think you may have been exposed to HIV, get an HIV test. Most HIV tests detect antibodies (proteins your body makes against HIV), not HIV itself. But it takes a few weeks for your body to produce these antibodies, so if you test too early, you might not get an accurate test result. A new HIV test is available that can detect HIV directly during this early stage of infection. So be sure to let your testing site know if you think you may have been recently infected with HIV.

What kind of tests are there?


Rapid HIV diagnostic home-kit tests are available, but these tests should be used with extreme care. It is not advisable to do the home test without proper pre-test and post-test counselling. The results can also be incorrect if the testing instructions are not followed exactly, if the test has not been stored at the required temperature, if the expiry date of the test has been exceeded, or if tests of a low quality are used. A positive result obtained from a home test should always be confirmed by a subsequent laboratory test. HIV self-tests are already available in some pharmacies for about R 35.


A rapid HIV antibody test is a blood test, which can be done by pricking the finger with a lancet, and applying the test tube. The result is ready within 15 minutes.

Rapid tests are very useful for the diagnosis of HIV infection in rural or isolated areas that are far removed from diagnostic laboratories and where clients often cannot afford to come back for test results.

These tests are relatively cheap and they demonstrate a high rate of reliability if they are correctly used.


Once a person becomes infected with HIV, a number of steps must take place before an HIV antibody test can detect infection. First, the virus infects more and more cells in the person’s body. The person’s immune system then responds by producing HIV antibodies, which are proteins that circulate in the blood to try to fight the HIV infection. It is these antibodies that the HIV antibody test detects, NOT HIV itself. If there aren’t enough antibodies created by the body when the test is given, the result will be negative despite the person being infected with HIV.

In order for an HIV antibody test to detect an HIV infection, two conditions must be met:

HIV antibodies must be present in the person’s blood. The amount of time it takes for the immune system to create HIV antibodies after HIV infection varies based on genetics, how transmission occurred (e.g. sex vs. shared needles), the amount of virus the person was exposed to, whether they underwent post-exposure prophylaxis (when someone takes a combination of anti-HIV medications within 72 hours of exposure to reduce the chances of becoming infected), and numerous other factors. For more on PEP, see Can we prevent infection with HIV after an exposure? The world of post-exposure prophylaxis (PEP).
The HIV antibody test must be sensitive enough to detect the antibodies. Newer testing technologies can now detect HIV antibodies when there are lower concentrations in the blood and hence are able to detect an HIV infection much sooner.
The “window period” refers to the maximum amount of time it may take for a person’s body to create HIV antibodies after HIV infection. HIV antibodies must be present in order for the HIV antibody test to accurately detect HIV antibodies in someone’s blood. If someone is “in the window period,” there is a chance that even though they may have been infected with HIV, the test won’t be able to detect the infection and will give a negative result.

HIV antibody tests are the most widely used HIV tests. One of the main reasons they are so widely used is that once HIV antibodies are present in a person’s blood, they will be there for the rest of that person’s life (provided their immune system is functioning properly). This means that following the window period, an HIV antibody test will always give a positive result for someone who has been infected with HIV. This is important as many people are not diagnosed until several years after infection.

Older HIV antibody tests were significantly less able than newer tests to detect low levels of HIV antibodies in a person’s blood, which is why in the past the window period was set at 6 months.

Research shows us that with these new tests, as many as 95% of people who test positive will do so within 34 days of exposure to HIV. However, for the remaining 5%, the window period for these HIV antibody tests is generally accepted to be three months so as to ensure people who take longer to develop antibodies are not overlooked. This means that if someone tests negative for HIV antibodies during the window period, they should be re-tested three months after possible exposure, to fully rule out HIV infection.

There are some rare exceptions which may require someone to retest up to six months after exposure:

People who have a severely impaired immune system may take longer to develop HIV antibodies.
People who have taken post-exposure prophylaxis (PEP). This is a one-month course of anti-HIV drugs that, if started within 72 hours of potential exposure, may reduce the risk of HIV infection. If PEP does not successfully prevent HIV infection in someone, the presence of the PEP drugs may still reduce HIV replication until the drugs are finished. This may delay the time it takes for a person’s body to create HIV antibodies.


Tests that directly detect HIV

There are a number of technologies that detect HIV itself. It is important to do some research and find out which testing services are available for your clients to help ensure effective messaging for someone who thinks they have recently been exposed to HIV.

The two most commonly used tests that detect HIV directly are the p24 antigen test and the HIV nucleic acid amplification test (NAAT).


  • The HIV p 24 antigen test, the most widely available of the two, is designed to detect a protein (the p 24 protein) associated with HIV. The p 24 antigen test can detect the p 24 protein on average 10 to 14 days after infection with HIV. One drawback of this test is that levels of the p 24 protein peak at around three to four weeks after exposure to HIV and are usually not detectable after five to six weeks (and sometimes even earlier). A positive p 24 test means that someone is HIV-positive. However, a negative p 24 test can mean three things:
  • the person is HIV-negative
  • the person is HIV-positive but that the test could not detect the p 24 protein because the person was infected more than four to six weeks earlier
  • the levels of p 24 antigen are too low to be detectable with current technologies.

The most advanced tests combine a p 24 antigen test and an antibody test. These tests are seen as beneficial because they combine the early detection abilities of the p 24 antigen test with the accuracy of the newer antibody tests. It should be noted that a rapid (point-of-care) version of these tests is not yet available.


The HIV NAAT (Nucleic acid amplification techniques) test is a very sensitive test designed to detect HIV RNA in blood. RNA is the viral equivalent to human DNA. The NAAT test is able to detect HIV RNA as early as seven to 14 days after infection with HIV. Unlike the p24 test, the NAAT test will always give a positive result as long as there is HIV in someone’s blood.

Seroconversion symptoms

It is also important to talk about the symptoms of seroconversion when we talk about testing.

Seroconversion is the interval, several weeks after HIV infection, during which antibodies are first produced and rise to detectable levels.

Antibodies generally begin to appear within one to two weeks of exposure, and antibody concentrations (titres) continue to increase for several months thereafter. Seroconversion takes place within three weeks in most infected individuals, although very rare cases are reported in which seroconversion does not occur for up to a year.

Seroconversion is often – although not always – accompanied by a flare of symptoms called seroconversion illness (sometimes also called acute retroviral syndrome). Symptomatic seroconversion illness occurs in at least 50%, and possibly as many as 80 or 90%, of infected individuals. The symptoms can occur from two to four weeks after infection and may include flu-like symptoms, such as fatigue, fever, sore throat, swollen lymph nodes, headache, loss of appetite or skin rash. This illness usually lasts less than two weeks although it can last as long as 10 weeks. Educating clients about the symptoms of seroconversion may increase the likelihood that they will get tested if they experience symptoms.

If a client has had a recent high-risk encounter and experiences any of these symptoms, they should be encouraged to have an HIV test. Depending on the time since infection, the antibody test may not give an accurate result. However, if available, the p 24 antigen test will be able to give an accurate result two to four weeks following infection and the NAAT test will be accurate in as little as seven to 14 days after infection.


What does all this mean for front-line workers?

The early detection of HIV is important because people who are newly infected are very infectious and may inadvertently transmit HIV to others. There is still a lot of misunderstanding about how soon one should get tested after potential HIV exposure. Many people still believe they have to wait three months. However, new and improved testing technologies are continually decreasing the amount of time it takes for a new HIV infection to be detected.

In the case of clients at high risk of HIV, testing can be done as early as one month after exposure for standard antibody assays and rapid point-of-care tests. Clients who test positive will know for certain they are HIV-positive. Of those who test negative, 95% are in fact negative. It is important to realize that up to 5% of people who test negative at one month could later test positive at three months. It is important to ensure that people testing negative at one month are advised to return for repeat testing once the three-month window period is covered.


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